Two RT-PCR based assays to detect human metapneumovirus in nasopharyngeal aspirates
نویسندگان
چکیده
منابع مشابه
Detection of Human Influenza Viruses in Nasopharyngeal Samples by RT-PCR vs Tissue Culture
Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...
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Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2005
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2005.05.004